Method of immobilizing enzymes or microbes with alginate having

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435182, C12N 1110, C12N 1104

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active

049506005

ABSTRACT:
Enzymes or microbes are immobilized by using low mannuronic acid to guluronic acid (M/G) ratio alginate to produce an alginate gel having improved strength. An aqueous solution containing an enzyme or microbe and alginate having an M/G ratio of 0.01-0.8 (preferably 0.01-0.3) is contacted with an aqueous solution of barium ion or strontium ion to gel the alginate.

REFERENCES:
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patent: 4663287 (1987-05-01), Barker
DE-Buch: Rehm, H. J., Reed, G.: Biotechnology, vol. 7a, Weinheim: VCH-Verlagsgesellschaft mbH, 1987.
F. Paul et al, "Enzyme Microb. Technol.", (1980), vol. 2, pp. 281-287.
A. L. Dainty et al, "Biotechnology and Bioengineering", (1986), vol. 28, pp. 210-216.
Biotechnology and Bioengineering, vol. 24, No. 7, 1982, pp. 1507-1517, M. Kierstan et al., "Studies on the Characteristics of Alginate . . . ".
Chemical Abstracts, vol. 83, No. 26, Dec. 29, 1975, pp. 97-98, Abstract No. 207815d, Seeley et al., "Binding of Alkaline Earth . . . ".

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