Method of immobilizing enzymatically active materials

Details Method of immobilizing enzymatically active materials Method of immobilizing enzymatically active materials

1983-04-22

1985-06-25

Rosen, Sam

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435179, 435180, 435181, C12N 1110, C12N 1108

Patent

active

045254575

ABSTRACT:
This invention discloses a method of immobilizing an enzymatically active material to a water-insoluble polyanion which comprises bringing a polycation-containing aqueous medium and the enzymatically active substance into contact with the water-insoluble polyanion having any desired form. This method is characterized in that the enzymatically active substance is adsorbed on the surfaces of the water-insoluble polyanion and, at the same time, a strong complex is formed by the interaction of the polycation and the polyanion to produce a water-insoluble film. As a result, the enzymatically active substance so adsorbed is securely fixed to the surfaces of the water-insoluble polyanion and scarcely released therefrom.

REFERENCES:
patent: 4194066 (1980-03-01), Kaetsu et al.

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