Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...
Reexamination Certificate
2008-09-30
2008-09-30
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
C435S176000, C435S177000, C435S195000, C530S402000
Reexamination Certificate
active
11006031
ABSTRACT:
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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Darzins Aldis
Encell Lance
Klaubert Dieter
Los Georgyi V.
McDougall Mark
Kosson Rosanne
Promega Corporation
Prouty Rebecca E.
Schwegman Lundberg & Woessner, P.A.
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