Method of imaging fluorescence and fluorescence imaging...

Details Method of imaging fluorescence and fluorescence imaging... Method of imaging fluorescence and fluorescence imaging...

2007-12-05

2009-10-13

Evans, F. L (Department: 2877)

Optics: measuring and testing

By shade or color

With color transmitting filter

C356S317000

Reexamination Certificate

active

07602494

ABSTRACT:
A method of imaging fluorescence, in which an inverted fluorescence imaging apparatus provided with an immersion objective is used to image an observation phosphor for a constant term, includes an imaging position locating step of locating a vessel incorporating the observation phosphor at an imaging position of the fluorescence imaging apparatus; a liquid pouring step of pouring a preset amount of liquid between a top portion of the immersion objective in the fluorescence imaging apparatus and the vessel to bring about a charged state; an imaging step of imaging the observation phosphor in the vessel in a state where a space between the top portion of the immersion objective and the vessel is charged with the liquid; and a repeated control step of repeating a series of operations including the liquid pouring step of a single operation and the imaging step of a preset number of times of operations performed after the liquid pouring step of the single operation, at preset time intervals.

REFERENCES:
patent: 6980293 (2005-12-01), Harada
patent: 2005/0022731 (2005-02-01), Petrillo et al.
patent: 2005/0105172 (2005-05-01), Hasegawa et al.
patent: 2008/0117421 (2008-05-01), Yamaguchi et al.
patent: 1717630 (2006-11-01), None
patent: 2004531765 (2004-10-01), None
patent: 2005234457 (2005-09-01), None
Partial European Search Report dated Apr. 10, 2008 in corresponding foreign application, 5 pp.

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