Method of identifying viruses

Details Method of identifying viruses Method of identifying viruses

1988-11-08

1991-12-24

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 29, 435 34, C12Q 170

Patent

active

050752146

ABSTRACT:
A method is described for identifying a virus infecting a host cell material, where the virus does not induce adequate or consistent host cell protein synthesis suppression, which comprises contacting a sample of the virus infected host cell material with an effective host cell protein synthesis suppression agent, allowing the virus to produce proteins characteristic of that virus in the host cell material following suppression of the host cell protein synthesis, analyzing the viral proteins so produced, obtaining from such analysis a record containing indicia uniquely characteristic of those viral proteins, and identifying the virus which produced the viral proteins by comparing the unique indicia with a library of predetermined like indicia, each of which has previously been identified with a specific virus. Preferably the host cell protein synthesis suppression agent is sodium chloride. Analysis of the viral proteins is preferably accomplished radiolabeling the proteins and separating them by electrophoresis, most preferably SDS-PAGE. The radiolabeled viral proteins can be identified by beta radiation counters or by autoradiography. The method rapidly and consistently identifies viruses to the genus, species or sub-species level, preferably by comparison to a database of like indicia using pattern recognition software.

REFERENCES:
patent: 4521512 (1985-06-01), Silman
Kozak, M., 1986, Regulation of Protein Synthesis in Virus Infected Animal Cells, Adv. Virus Res. 31 229.
Garry, R. F. et al., 1979, Na.sup.+ and K.sup.+ Concentrations and the Regulation of Protein Synthesis in Sindbis . . . , Virology 96 108.
Dunbar, B. S., 1987, 2 Dimensional Electrophoresis and Immunological Techniques, Plenum Press, N.Y., pp. 293-294.
Doel, Peptide Analysis of Viral Proteins, New Dev. in Practical Virology, 143-183 (1982).
Hook et al., Automated Microbial Identification System for Computer-Programmer Analysis of Radiolabeled Protein Banding Patterns, Developments in Industrial Microbiology, vol. 28 (1987).
Laemmli, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophase T4, Nature, 227, pp. 680-685 (Aug. 15, 1970).
Walpita et al., Abstract Form: Poster Session, presented at 7th International Symposium on Medical Viroloby in Anaheim, Calif. on Nov. 12, 1988.
Walpita et al., 1988 ASM Annual Meeting, presented at American Society for Microbiology in Miami, Fla., during week of May 8, 1988.

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