Method of identifying potential fungicides using dihydroorotate

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435220, C12N 900

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active

059768487

ABSTRACT:
Dihydroorotate dehydrogenase (DHOD) inhibititon assays are utilized in a method for identifying new fungicides; DHOD inhibitors are useful in fungicidal methods and compositions. Resistance genes that are mutants of the wild-type Aspergillus nidulans dihydroorotate dehydrogenase gene have been isolated and found to impart resistance to certain DHOD inhibitors. The resistance genes are useful as selectable markers in fungi. The wild-type gene is also provided.

REFERENCES:
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Smith, A.J. et al., Phytopathology 80:1053; Poster presented at the Annual Meeting of the American Phytopathological Society; Aug. 4-9, 1990; Grand Rapids, MI.
Gustafson et al., Curr. Microbiol. 23:39 (1991).

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