Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-05-13
2000-04-11
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 911, 435 912, 435 915, 435 9121, 435 18, 530350, 536 231, 536 243, C12Q 168, C12P 1934, C07M 2102, C07K 1300
Patent
active
060486961
ABSTRACT:
A method of analyzing a DNA molecule is disclosed. In one embodiment the method comprises the steps of exposing a DNA molecule to an effective amount of a chemical modification reagent wherein the reagent converts guanine to 8-hydroxyguanine. The oxidized product is then exposed to a DNA glycosylase enzyme and the DNA molecule is cleaved at the site of the 8-hydroxyguanine. The fragments are then resolved by electrophoresis and the position of guanine residues within the DNA molecule is determined. In a preferred embodiment of the present invention, the modification reagent is a thiazine dye and the enzyme is FPG protein.
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Hawkins Gregory A.
Hoffman Leslie M.
Epicentre Technologies Corporation
Fredman Jeffrey
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