Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-02-07
2006-02-07
Chan, Christina (Department: 1644)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007210, C435S069100, C435S069700, C435S325000, C435S320100
Reexamination Certificate
active
06994977
ABSTRACT:
The present invention relates to a method of identifying an inhibitor of a mammalian (e.g., human) C—C chemokine receptor 3 (CCR3), comprising combining (a) a compound to be tested, (b) a host cell expressing a recombinant protein comprising a mammalian CCR3, and (c) a ligand of said receptor, under conditions suitable for binding of ligand to said receptor, and detecting or measuring the formation of a receptor-ligand complex. Inhibition of complex formation by the compound is indicative that the compound is an inhibitor.
REFERENCES:
patent: 5225538 (1993-07-01), Capon et al.
patent: 5284746 (1994-08-01), Sledziewski et al.
patent: 5470571 (1995-11-01), Herlyn et al.
patent: 5652133 (1997-07-01), Murphy
patent: 6271347 (2001-08-01), Daugherty et al.
patent: 6537764 (2003-03-01), Gerard et al.
patent: 4332256 (1993-09-01), None
patent: 0475746 (1992-03-01), None
patent: 0578962 (1994-01-01), None
patent: WO 92/01810 (1992-02-01), None
patent: WO 94/05695 (1994-03-01), None
patent: WO 94/11504 (1994-05-01), None
patent: WO 94/12635 (1994-06-01), None
patent: WO 94/20142 (1994-09-01), None
patent: WO 94/28931 (1994-12-01), None
patent: WO 95/08576 (1995-03-01), None
patent: WO 95/31467 (1995-11-01), None
patent: WO 96/22371 (1996-07-01), None
patent: WO 97/21812 (1997-06-01), None
patent: WO 97/22698 (1997-06-01), None
patent: WO 97/41154 (1997-11-01), None
Springer, T.A., “Traffic Signals for Lymphocyte Recirculation and Leukocyte Emigration: The Multistep Paradigm,”Cell, 76:301-314 (1994).
Murphy, P.M., “The Molecular Biology of Leukocyte Chemoattractant Receptors,”Annu. Rev. Immunol., 12:593-633 (1994).
Baggiolini, M. and Dahinden, C.A., “CC Chemokines in Allergic Inflammation,”Immunology Today, 15(3):127-133 (1994).
Gerard N.P. and Gerard, C., “The Chemotactic Receptor for Human C5a Anaphylatoxin,”Nature, 349:614-617 (1991).
Neote, K. et al., “Molecular Cloning, Functional Expression, and Signaling Characteristics of a C-C Chemokine Receptor,”Cell, 72:415-425 (1993).
Gao, J-L. et al., “Structure and Functional Expression of the Human Macrophage Inflammatory Protein 1α/RANTES Receptor,”J. of Exp. Med., 177:1421-1427 (1993).
Charo, I.F. et al., “Molecular Cloning and Functional Expression of Two Monocyte Chemoattractant Protein 1 Receptors Reveals Alternative Splicing of the Carboxyl-Terminal Tails,”Proc. Natl. Acad. Sci. USA, 91:2752-2756 (1994).
Van Riper, G. et al., “Induction, Characterization, and Functional Coupling of the High Affinity Chemokine Receptor for RANTES and Macrophage Inflammatory Protein-1α upon Differentiation of an Eosinophilic HL-60 Cell Line,”J. of Immunol., 152:4055-4061 (1994).
Bischoff, S.C. et al., “RANTES and Related Chemokines Activate Human Basophil Granulocytes Through Different G Protein-Coupled Receptors,”Eur. J. Immunol., 23:761-767 (1993).
Dahinden, C.A. et al., “Monocyte Chemotactic Protein 3 is a Most Effective Basophil- and Eosinophil-activation Chemokine,”J. Exp. Med., 179:751-756 (1994).
Lefkowitz, R.J., “Turned on to Ill Effect,”Nature, 365:603-604 (1993).
Clapham, D.E., “Mutation in G Protein-Linked Receptors: Novel Insights on Disease,”Cell, 75:1237-1239 (1993).
Ponath, P.D., “C—C Chemokine Receptor 3: Identification of a Major Eosinophil Chemotactic Cytokine Receptor,” In: Conference Schedule for Conference Entitled “On the Cutting Edge of Anti-Inflammatory Drug Discovery,” (Jan. 1995).
Combadiere, C. et al., “Cloning and Functional Expression of a Human Eosinophil CC Chemokine Receptor,”J. of Biol. Chem., 270(27):16491-16494 (1995).
Combadiere, C. et al., Correction “Cloning and Functional Expression of a Human Eosinophil CC Chemokine Receptor,”J. Biol. Chem., 270:30235 (1995).
Post, T.W. et al., “Molecular Characterization of Two Murine Eosinophil β Chemokine Receptors,”J. of Immunol., 155:5299-5305 (1995).
Gao, J-L. and Murphy, P.M., “Cloning and Differential Tissue-specific Expression of Three Mouse β Chemokine Receptor-like Genes, Including the Gene for a Functional Macrophage Inflammatory Protein-1α Receptor,”J. of Biol. Chem., 270(29):17494-17501 (1995).
Ponath, P.D. et al., “Molecular Cloning and Characterization of a Human Eotaxin Receptor Expressed Selectively on Eosinophils,”J. Exp. Med., 183:2437-2448 (1996).
Daugherty, B.L. et al., “Cloning, Expression, and Characterization of the Human Eosinophil Eotaxin Receptor,”J. Exp. Med., 183:2349-2354 (1996).
Kitaura, M. et al., “Molecular Cloning of Human Eotaxin, an Eosinophil-Selective CC Chemokine, and Identification of a Specific Eosinophil Eotaxin Receptor, CC Chemokine Receptor 3,”J. of Biol. Chem., 271(13):7725-7730 (1996).
Jose, P.J. et al., “Eotaxin: A Potein Eosinophil Chemoattractant Cytokine Detected in a Guinea Pig Model of Allergic Airways Inflammation,”J. Exp. Med., 179:881-887 (1994).
Förster, R. et al., “A General Method for Screening mAbs Specific for G-Protein Coupled Receptors as Exemplified by Using Epitope Tagged BLR1-Transfected 293 Cells and Solid-Phase Cell ELISA,”Biochem. and Biophys. Res. Comm., 196(3):1496-1503 (1993).
Ponath, P.D. et al., “Cloning of the Human Eosinophil Chemoattractant, Eotaxin Expression, Receptor Binding, and Functional Properties Suggest a Mechanism for the Selective Recruitment of Eosinophils,”Jour. of Clinical Invest., 97(3):604-612 (1996).
Szigeti, V., and Miller R.H., “A Cell Surface Antigen Expressed by Astrocytes and Their Precursors,”GLIA, 8:20-32 (1993).
Gillaspy, G.E. et al., “Antigenic and Differentiative Heterogeneity Among Human Glioblastomas,”Cancer Letters, 68:215-224 (1993).
Lyon, J.A. et al., “Monoclonal Antibody Characterization of the 195-Kilodalton Major Surface Glycoprotein ofPlasmodium falciparumMalaria Schizonts and Merozoites: Identification of Additional Processed Products and a Serotype-Restricted Repetitive Epitope,”J. Immunol., 138(3):895-901 (1987).
Forssmann, U. et al., “Eotaxin-2, a Novel CC Chemokine that is Selective for the Chemokine Receptor CCR3, and Acts Like Eotaxin on Human Eosinophil and Basophil Leukocytes,”J. Exp. Med., 185(12):2171-2176 (1997).
Heath, H. et al., “Chemokine Receptor Usage by Human Eosinophils,”J. Clin. Invest., 99(2):178-184 (1997).
Yamagami, S. et al, “cDNA Cloning and Functional Expression of a Human Monocyte Chemoattractant Protein 1 Receptor,”Biochemical Biophysical Research Communications, 202(2):1156-1162 (1994).
Sabroe, I. et al., “Cloning and Characterization of the Guinea Pig Eosinophil Eotaxin Receptor, C—C Chemokine Receptor-3: Blockade Using a Monoclonal Antibody In Vivo,”The Journal of Immunology, 161:6139-6147 (1998).
Henderson, B. et al., “Therapeutic Potential of Cytokine Manipulation,”Trends Pharmacol Sci, 13(4):145-152 (1992).
Chaudhuri, A. et al., “Expression of the Duffy Antigen in K562 Cells. Evidence that it is the Human Erythrocyte Chemokine Receptor,”J. Biol. Chem., 269(11):7835-7838 (1994).
Ngo, J.T. et al., “Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox,” In:The Protein Folding Problem and Tertiary Structure Prediction, Merz & LeGrand, Eds. (Birkhauser, Boston), pp. 491-495 (1994).
Neote, K. et al., “Molecular Cloning, Functional Expression, and Signaling Characteristics of a C-C Chemokine Receptor,”Cell, 72:415-425 (1993).
Navia, M.A. et al., “Structure-Based Drug Design: Applications in Immunopharmacology and Immunosuppression,”Immunology Today, 14:295-301 (1993).
GenEmbl Accession No. L09230, Human C—C Chemokine Receptor Type 1 (C_CCKR-1) mRNA, complete CDS (1994).
Murphy, P.M., et al., “International Union of Pharmacology. XXII. Nomenclat
Gerard Craig J.
Gerard Norma P.
Mackay Charles R.
Ponath Paul D.
Post Theodore W.
Brigham & Women's Hospital
Chan Christina
Children's Medical Center Corporation
DiBrino Marianne
Hamilton Brook Smith & Reynolds P.C.
LandOfFree
Method of identifying inhibitors of C—C chemokine... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of identifying inhibitors of C—C chemokine..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of identifying inhibitors of C—C chemokine... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3686069