Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-10-02
2000-08-08
McKelvey, Terry
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 168
Patent
active
061000353
ABSTRACT:
The invention provides a method of identifying nucleic acid molecules that contain cis acting nucleic acid elements. Also provided is a method of isolating nucleic acid binding factors. The invention also provides methods of identifying compounds that are cis acting nucleic acid element analogs, compounds that are nucleic acid binding factor analogs, compounds that selectively bind cis acting nucleic acid elements and compounds that selectively displace binding between a nucleic acid binding factor and a cis acting nucleic acid element or between nucleic acid binding factors. Also provided is a method of determining a binding state of a nucleic acid. Pluralities of isolated nucleic acid molecules containing cis acting nucleic acid elements, of isolated cis acting nucleic acid elements and of isolated nucleic acid binding factors are also provided. The invention further provides methods of treating pathological conditions using molecules of the invention to alter genetic activities of nucleic acids involved in pathological conditions.
REFERENCES:
patent: 5270163 (1993-12-01), Gold et al.
patent: 5306619 (1994-04-01), Edwards et al.
patent: 5567588 (1996-10-01), Gold et al.
patent: 5578444 (1996-11-01), Edwards et al.
patent: 5582981 (1996-12-01), Toole et al.
patent: 5595877 (1997-01-01), Gold et al.
patent: 5637459 (1997-06-01), Burke et al.
patent: 5670637 (1997-09-01), Gold et al.
patent: 5683867 (1997-11-01), Biesecker et al.
patent: 5688935 (1997-11-01), Stephens et al.
patent: 5693463 (1997-12-01), Edwards et al.
patent: 5696249 (1997-12-01), Gold et al.
patent: 5705337 (1998-01-01), Gold et al.
patent: 5712375 (1998-01-01), Jensen et al.
patent: 5723289 (1998-03-01), Eaton et al.
patent: 5723592 (1998-03-01), Eaton et al.
patent: 5726014 (1998-03-01), Edwards et al.
patent: 5750342 (1998-05-01), Stephens et al.
patent: 5763177 (1998-06-01), Gold et al.
patent: 5763566 (1998-06-01), Jensen et al.
patent: 5763595 (1998-06-01), Gold et al.
patent: 5773598 (1998-06-01), Burke et al.
patent: 5789157 (1998-08-01), Jensen et al.
patent: 5789160 (1998-08-01), Eaton et al.
patent: 5817785 (1998-10-01), Gold et al.
patent: 5843653 (1998-12-01), Gold et al.
patent: 5853984 (1998-12-01), Davis et al.
patent: 5858660 (1999-01-01), Eaton et al.
patent: 5861246 (1999-01-01), Weisman et al.
patent: 5861254 (1999-01-01), Schneider et al.
patent: 5864026 (1999-01-01), Jensen et al.
patent: 5874218 (1999-02-01), Drolet et al.
Grossman et al., "The use of antibodies to the polypyrimidine tract binding protein (PTB) to analyze the protein components that assemble on alternatively spliced pre-mRNAs that use distant branch points," RNA 4:613-625 (1998).
Yu et al., "Specific binding of host cellular proteins to multiple sites within the 3' end of mouse hepatitis virus genomic RNA," Journal of Virology 69:2016-2023 (1995).
Blackwell, "Selection of Protein Binding Sites from Random Nucleic Acid Sequences," Methods in Enzymology 254:604-618 (1995).
Ellington and Szostak, "In vitro selection of RNA molecules that bind specific ligands," Nature 346:818-822 (1990).
Funk and Wright, "Cyclic amplification and selection of targets for multicomponent complexes: Myogenin interacts with factors recognizing binding sites for basic helix-loop-helix, nuclear factor 1, myocyte-specific enhancer binding factor 2, and COMP1 factor," Proc. Natl. Acad. Sci. USA 89:9484-9488 (1992).
Hagenbuchle and Wellauer, "A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells in culture," Nucleic Acids Research 20:3555-3559 (1992).
Mavrothalassitis et al., "Defining Target Sequences of DNA-Binding Proteins by Random Selection and PCR: Determination of the GCN4 Binding Sequence Repertoire," DNA and Cell Biology 9:783-788 (1990).
Nallur et al., "Multiplex selection technique (MuST): An approach to clone transcription factor binding sites," Proc. Natl. Acad. Sci. USA 93:1184-1189 (1996).
N.o slashed.rby et al., "Determination of recognition-sequences for DNA-binding proteins by a pollymerase chain reaction assisted binding site selection method (BSS) using nitrocellulose immobilized DNA binding protein," Nucleic Acids Research 20:6317-6321 (1992).
Pollock and Treisman, "A sensitive method for the determination of protein-DNA binding specificities," Nucleic Acids Research 18:6197-6204 (1990).
Wright and Funk, "CASTing for multicomponent DNA-binding complexes," TIBS 18:77-80 (1993).
Wright et al., "Cyclic Amplification and Selection of Target (CASTing) for the Myogenin Consensus Binding Site," Molecular and Cellular Biology 11:4104-4110 (1991).
Duzgunes and Felgner, "Intracellular Delivery of Nucleic Acids and Transcription Factors by Cationic Liposomes," Methods In Enzymology, 221:303-306 (1993).
Fitzwater and Polisky, A SELEX Primer, "A SELEX Primer," Nucleic Acid Libraries, Chapter 17, pp. 275-301 (1996).
Gold et al., "Diversity of Oligonucleotide Functions," Annu. Rev. Biochem., 64:763-797 (1995).
Gold et al., "From Oligonucleotide Shapes to Genomic SELEX: Novel Biological Regulatory Loops," Proc. Natl. Acad. Sci. USA, 94:59-64 (1997).
Gold et al., "SELEX and the Evolution of Genomes," Current Opinion in Genetics & Development, 7:848-851 (1997).
Hardenbol et al., "Identification of Preferred hTBP DNA Binding Sites by the Combinatorial Method REPSA," Nucleic Acids Research, 25:3339-3344 (1997).
Isalan et al., "Comprehensive DNA Recognition through Concerted Interactions from Adjacent Zinc Fingers," Biochemistry, 37:12026-12033 (1988).
Kinzler and Bogelstein, "Whole Genome PCR: application to the identification of sequences bound by gene regulatory proteins," Nucleic Acids Research, 17(10):3645-3653 (1989).
Morishita et al., "Application of Transcription Factory "Decoy" Strategy as Means of Gene Therapy and Study of Gene Expression in Cardiovascular Disease," Cir Res, 82:1023-1028 (1998).
Morris et al., "High affinity ligands from in vitro selection: Complex targets," Proc. Natl. Acad. Sci. USA, 95:2902-2907 (1988).
Nallur et al., "Multiplex Selection Technique (MuST): An Approach to Clone Transcription Factor Binding Sites," Proc. Natl. Acad. Sci. USA, 93:1184-1189 (1996).
Oliphant et al., "Cloning of random-sequence oligodeoxynucleotides," Gene, 44:177-183 (1986).
Oliphant et al., "Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein," Mol. and Cell. Biol., 9(7):2944-2949 (1989).
Oliphant, A. et al., "The Use of Random-Sequence Oligonucleotides for Determining Consensus Sequences," Methods in Enzymology, 155:568-582 (1987).
Rebar and Pabo, "Zinc Finger Phage: Affinity Selection of Fingers with New DNA-Binding Specificities," Science, 263:671-673 (1994).
Singer et al., "Libraries for genomic SELEX," Nucleic Acids Research, 25(4):781-786 (1997).
Sullenger et al., "Analysis of trans-Acting Response Decoy RNA-Mediated Inhibition of Human Immunodeficiency Virus Type 1 Transactivation," J. of Virology, 65(12):6811-6816 (1991).
Thiesen and Bach, "Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein," Nucleic Acids Research, 18(11):3203-3209 (1990).
Tuerk, Craig, "Using the SELEX Combinatorial Chemistry Process to Find High Affinity Nucleic Acid Ligands to Target Molecules," 67:219-230 (19!!).
Vant-Hull et al., "The Mathematics of SELEX Against Complex Targets," J. Mol. Biol., 278:579-597 (1998).
Ballivet Marc
Kauffman Stuart A.
Cistem Molecular Corporation
McKelvey Terry
LandOfFree
Method of identifying cis acting nucleic acid elements does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of identifying cis acting nucleic acid elements, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of identifying cis acting nucleic acid elements will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1148876