Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-08-06
1999-08-31
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 34, G01N 33567, C12Q 102, C12Q 104
Patent
active
059452927
ABSTRACT:
This invention discloses a gene for the identification of cells comprising a secretion leader segment, a cell marker segment and a transmembrane segment. The gene can be used to identify cells transfected with the gene by the steps of: inserting the gene having a secretion leader segment, a cell marker segment and a transmembrane segment into the DNA or RNA of a cell, allowing the cell to express the gene, and detecting the expressed cell marker segment of the gene.
REFERENCES:
patent: 4650756 (1987-03-01), Old et al.
patent: 4703004 (1987-10-01), Hopp et al.
patent: 4782137 (1988-11-01), Hopp et al.
patent: 4810632 (1989-03-01), McMillan
patent: 4851341 (1989-07-01), Hopp et al.
patent: 4978621 (1990-12-01), Ardeshir et al.
patent: 5011912 (1991-04-01), Hopp et al.
patent: 5124251 (1992-06-01), Lanier et al.
patent: 5185438 (1993-02-01), Lemischka
patent: 5242822 (1993-09-01), Marullo et al.
patent: 5270458 (1993-12-01), Lemischka
patent: 5336597 (1994-08-01), McMillan
Bianca et al. Mol. Biol. Cell 4 Suppl. 192 A Abs. 1121, 1993.
Orkin et al. Report & Recommendation of the Panel to Assess the NIH Investment in Research on Gene Therapy, 1995.
Kriegler, M., Gene Transfer and Expression a Laboratory Manual, W.H. Freeman and Company, New York (1990).
Wigler et al., Cell, vol. 11, p. 223 (1977).
Southern et al., J. Mol Appl. Gen. vol. 1, p. 327 (1982).
Padmanabhan, et al., Methods in Enzymology, vol. 218, pp. 637-651 (1993).
Binaca et al. Use of the Flag.RTM. Epitope to Examine Expression and Function of a Synthetic Gene Encoding a Cell Surface Protein in Mammalian Cells, Mol. Biol. Cell. 4 (Suppl) #121, Dec. 1993 Abstract.
Bianca Darlene W.
Brizzard Billy L.
Chubet Richard G.
Hopp Thomas Patrick
Vizard Douglas L.
Degen Nancy
Sigma-Aldrich Co.
LandOfFree
Method of identifying cells with polypeptide surface marker does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of identifying cells with polypeptide surface marker, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of identifying cells with polypeptide surface marker will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2426328