Method of identification and preparation of probes for pestiviru

Details Method of identification and preparation of probes for pestiviru Method of identification and preparation of probes for pestiviru

1990-06-12

1992-05-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 91, 536 26, 536 27, 536 28, 536 29, 935 77, 935 78, 436 94, 436501, C12Q 170, C12Q 168, C12P 1934, C07H 1512

Patent

active

051127539

ABSTRACT:
In the method of identification and preparation of general and/or specific probes for varieties of Pestivirus, a length of approximately 110 to 115 bases, towards the 5' end on the Pestivirus genome in the non-coding region corresponding to the region situated between bases 200 and 310 of the genome of the BVD Osloss virus, is amplified by means of converging primers in a polymerase chain reactions, the amplified length is sequenced and, by comparison with similar previously sequenced lengths of other varieties of Pestivirus, the homologous and/or specific oligonucleotidic sequences of the probes are identified.

REFERENCES:
Collett et al., Virology 165: 191-199 (1988).
Saiki et al., Science 230: 1350-1354 (Dec. 1985).
Urdea, Gene 61: 253-264 (1987).

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