Method of human cell culture

Details Method of human cell culture Method of human cell culture

1982-05-21

1984-08-28

Wiseman, Thomas

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435948, C12N 500, C12R 191

Patent

active

044684606

ABSTRACT:
A method of culture of human cells is disclosed which comprises effecting the cultivation in a culture medium containing an extract of micro algae, such as Chlorella, Scenedesmus or Spirulina, said method permitting the normal successive cultivation of human cells to be maintained efficiently without any morphological and genetic mutations over a greater number of successive of generations than has hitherto been possible even by the incorporation of animal serum in the culture medium, even when the addition amount of such animal serum is reduced substantially or animal serum is completely excluded.

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patent: 4431738 (1984-02-01), Maeda et al.
Hayflick et al. "The Serial Cultivation of Human Diploid Cell Strains" Experimental Cell Research 25 (1961) p. 585-621.
Jakoby et al., "Cell Culture" Methods in Enzymology vol. LVIII (1979) p. 88-93.
Takada et al., "The Growth Promotive Effect of Extract from Saline-Cultured Chlorella Cells on Halophilic Microorganisms".
Seaweed Symposium Proc. 7 (1972) p. 324-335.
Practical Tissue Culture Applications, (1979), edited by Maramorosch and Hirumi, p. 29.
Botonay, (Fourth Edition), Wilson and Loomis, p. 400.
Cell Culture, (1979), edited by Jakoby, pp. 299-300.
Industrial Gums, (1973), edited by Whistler, pp. 30 and 87.

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