Method of growing sperm stem cells in vitro, sperm stem...

Details Method of growing sperm stem cells in vitro, sperm stem... Method of growing sperm stem cells in vitro, sperm stem...

2004-03-31

2010-02-23

Ton, Thaian N (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of co-culturing cells

C435S325000, C435S366000, C435S383000, C435S384000, C435S392000

Reexamination Certificate

active

07666673

ABSTRACT:
The present invention provides a method of growing spermatogonial stem cells of mammals and the like in vitro, which is characterized in that glial cell-derived neurotrophic factor (GDNF) or an equivalent thereto, and leukemia inhibitory factor (LIF) are contained in a medium (culture broth) for culturing spermatogonial stem cells. According to the method of the present invention, spermatogonial stem cells can be grown in vitro to the extent that enables use thereof for developmental engineering.

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