Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-10-01
1999-09-14
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4352884, C12Q 124
Patent
active
059521910
ABSTRACT:
A method and apparatus for growth and analysis of microbiological clones. A divider (6) is introduced into a layer of solid growth medium, separating the open surface of the medium into compartments. Appropriately diluted microbiological samples containing known strains of bacteria, yeast or virus infected bacteria are spread around the surface of the compartments. Glass beads can be used for spreading. Means for collecting beads contained within multiple compartments are provided. The divider is removed; means for preserving the structural integrity of the solid medium layer upon removal of the divider are provided. Colonies (in case of bacteria) or viral plaques (in case of virus infected bacteria) are allowed to form and subjected to further analyzes such as replica-plating or membrane lifts, which can now be performed on several samples simultaneously.
REFERENCES:
patent: 3677904 (1972-07-01), Fitzgerald
patent: 3715280 (1973-02-01), Farmer
patent: 3769936 (1973-11-01), Swanson
patent: 3796638 (1974-03-01), Guigan
patent: 3912596 (1975-10-01), Hague et al.
patent: 4042463 (1977-08-01), Haque
patent: 4053362 (1977-10-01), Sforza
patent: 4072578 (1978-02-01), Cady
patent: 4988302 (1991-01-01), Smith et al.
patent: 5262326 (1993-11-01), Jaeger
patent: 5358871 (1994-10-01), Stevens
MacFaddin, J. Media for Isolation, Cultivation, Identification, Maintenance of Medical Bacteria, Williams & Wilkins, Baltimore, 1985.
Poppe C. Evaluation of Immunomagnetic Separation in Combination with Modified Semi-Solid Rappaport Vassiliadis Medium and Rambach Agar for the Isolation of Salmonella, J of Microbiological Methods 25 237-244 1996.
Senthuran A. Lactic Acid Fermentation in a Recycle Batch Reactor Using Immobilized Lactobacillus casei, Biotechnology and Bioengineering 55(6) 841-853, Sep. 27, 1997.
LandOfFree
Method of growing several samples of microorganisms on a single does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of growing several samples of microorganisms on a single , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of growing several samples of microorganisms on a single will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1509195