Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Insect cell – per se
Patent
1995-08-25
1999-02-09
Housel, James C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Insect cell, per se
435374, 435395, 435400, 435243, 435248, 4352536, 435260, 4242651, C12N 100
Patent
active
058693351
ABSTRACT:
The invention is directed to methods of culturing rickettsiae in Ixodes scapularis cell lines. The methods of the invention provide for culture of microorganisms such as Anaplasma marginale, Ehrlichia canis, and Rickettsia rickettsii. A method of the invention involves incubating a rickettsia with an Ixodes scapularis tick cell culture in a culture medium under reduced oxygen and increased CO.sub.2 at a sufficient temperature until growth of the rickettsia is detected. The culture medium comprises a medium suitable for the growth of invertebrate cells supplemented with an organic buffer. The cell culture method can be used in large scale production of rickettsia containing products useful in diagnostic assays and vaccine preparations.
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Blouin Edmour F.
Ewing Sidney A.
Kocan Katherine M.
Kurtti Timothy J.
Munderloh Ulrike G.
Housel James C.
Regents of the University of Minnesota
Shaver Jennifer
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