Method of growing anchorage-dependent cells

Details Method of growing anchorage-dependent cells Method of growing anchorage-dependent cells

1980-08-27

1982-06-15

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435286, 435296, 435 41, 435 68, 435811, C12N 502, C12M 302, C12M 124, C12P 100

Patent

active

043352154

ABSTRACT:
Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor.

REFERENCES:
patent: 2967107 (1961-01-01), Geiger et al.
patent: 3234026 (1966-02-01), Coutts et al.
patent: 3591460 (1971-07-01), Smith
patent: 3647633 (1972-03-01), Dawson
patent: 3717551 (1973-02-01), Bizzini et al.
patent: 3880716 (1975-04-01), Engelbart et al.
patent: 4036693 (1977-07-01), Levine et al.
patent: 4189535 (1980-02-01), Levine et al.
van Hemert et al., Homogeneous Cultivation of Animal Cells for the Production of Virus and Virus Products, Biotech. Bioeng., vol. XI, 1969, 875-885.
Bulletin 1068, "Bio-Carriers.TM. for Cell Culture", Bio-Rad Labs, 1979, 4 pages.
Bulletin "Cytodex .TM.1-Beaded Microcarriers for Cell Culture", pp. 5 & 18, Pharmacia Fina Chemicals, May 1, 1978.
Bulletin "Superbead .TM.Microcarriers-Instructions for Use", Flow Laboratories, Inc., Nov. 1978, 2 pages.
Jakoby et al., Methods in Enzymology, vol. LVIII, Cell Culture, 1979, pp. 204, 209, 210.

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