Method of growing a confluent layer of cells on a porous or semi

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435284, 435285, 435297, C12N 500, C12M 300

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active

046083421

ABSTRACT:
A method and apparatus are disclosed for growing a confluent layer of cells on the surface of a substrate which is held by a holding and positioning device so as to be in the form of a taut, planar substrate having upper and lower exposed surfaces. The device is arranged in a nutrient-containing receptacle which also contains a plate material having a solid upper planar surface extending above the plane of the bottom of the receptacle. The lower layer of the receptacle is in contact with the upper surface of the plate material and cells disposed on the upper surface of the substrate are found to anchor to the substrate and grow to a confluent layer.

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