Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1989-01-31
1990-05-15
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041832, 204299R, B01D 5702, G01N 2728
Patent
active
049255452
ABSTRACT:
The present invention provides an improved method for forming pH gradients or other pH functions for use in electrophoresis and isoelectric focusing. It also involves electrophoresis and IEF devices created by such methods, and improved reagents suitable for generating such devices. The method involves (1) chemically affixing or "anchoring," in a concentration gradient or other varying function, selected molecules which form acidic complexes when contacted by certain acidic compounds, and (2) contacting the anchored molecules with a fluid containing a suitable acidic compound. One example of a paired combination of anchored molecules and acid comprises (a) anchored molecules with polyhydroxyl groups and (b) boric acid, borax, and/or other borate constituents. The borate will form acidic complexes with the anchored polyhydroxyl groups, generating pH's which will vary within the device depending on (1) the concentration and type of polyhydroxyl groups that are anchored in the device and (2) the composition and pH of the borate buffer.
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Amest, Inc.
Niebling John F.
Starsiak Jr. John S.
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