Method of generating neurons from stem cells and medium for...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology

Reexamination Certificate

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C435S368000, C435S325000, C435S373000, C435S404000, C435S405000

Reexamination Certificate

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10318997

ABSTRACT:
The present invention relates to a method of generating neurons from stem cells which comprises culturing neurons in a medium and culturing the stem cells in the resultant mixture. The present invention also relates to a medium for culturing stem cells prepared by culturing neurons in a base culture medium.

REFERENCES:
patent: 2003/0013192 (2003-01-01), Laeng et al.
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Cao, Q. L., et al. “Pluripotent Stem Cells Engrafted into the Normal or Lesioned Adult Rat Spinal Cord are Restricted to a Glial Lineage”,Experimental Neurology, (2001), 167: 48-58.
Sanchez-Ramos, J. R., et al. “Expression of Neural Markers in Human Umbilical Cord Blood”,Experimental Neurology, (2001), 171: 109-115.
Ha, Y., et al. “Neural phenotype expression of cultured human cord blood cells in vitro”,Neuroreport, (Nov. 2001), 12(16): 3523-3527.
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Azizi, S. A., et al. “Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats-similarities to astrocyte grafts”,Proc. Natl. Acad. Sci. USA, (Mar. 1998), 95: 3908-3913.
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