Method of generating multiple protein variants and populations o

Details Method of generating multiple protein variants and populations o Method of generating multiple protein variants and populations o

1994-04-28

1996-05-28

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 43525233, C12N 1500

Patent

active

055210775

ABSTRACT:
A method based on generalized recombination is provided for generating multiple protein variants. The method involves introducing into host cells at least two pools of mutant encoding nucleotide sequences, recipient and donor, transducing the donor pool into the recipient cells and subsequently screening and selecting the recombinants thereby produced for expressed proteins exhibiting desired characteristics. A population of such protein variants is also provided.

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