Method of generating a transgenic livestock animal

Details Method of generating a transgenic livestock animal Method of generating a transgenic livestock animal

2007-04-03

2007-04-03

Bertoglio, Valarie (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

Via microinjection of dna into an embryo, egg cell, or...

C800S014000, C800S015000, C800S016000, C800S017000

Reexamination Certificate

active

10238042

ABSTRACT:
The present invention provides methods of producing transgenic livestock animals. The methods generally involve first introducing a nucleoprotein made up of nucleic acid and a recombinase into a totipotent or pluripotent cell to produce a recombinant totipotent or pluripotent cell and then growing the recombinant totipotent or pluripotent cell to produce the transgenic livestock animal. The invention further provides kits for use in generating transgenic non-human animals of the invention.

REFERENCES:
patent: 5763240 (1998-06-01), Zarling et al.
patent: 5948653 (1999-09-01), Pati et al.
patent: 6074853 (2000-06-01), Pati et al.
patent: 6200812 (2001-03-01), Pati et al.
patent: 6255113 (2001-07-01), Zarling et al.
patent: WO 99/60108 (1999-11-01), None
Maga, E. The use of recombinase proteins to generate transgenic large animals, Cloning and Stem Cells, 3:233-241, 2001.
Niemann, 1997, Transg. Res. vol. 7, pp. 73-75.
Kilby, 1993, Trends in Genetics, 9:413-421.
Moreadith, 1997, Gene targeting in embryonic stem cells : the new physiology and metabolism, Journal of Molecular Medicine, vol. 75, pp. 208-216.
Seamark, 1994, Reproductive Fertility and Development, 6:653-7.
Mullins, 1996, J. Clin. Invest., vol. 98, pp. S37-S40.
McCreath, 2000, Nature, vol. 405, pp. 1066-1069.
Denning, C. 2001A, Nature Biotechnology, vol. 19, pp. 559-562.
Dinnyes, 2002, Cloning and Stem Cells, vol. 4, pp. 81-90.
Denning, C., 2001B, Gene Targeting from primaty fetal fibroblasts from sheep and pig, Cloning and Stem Cells, 3:221-231.
Poljaeva, I.A., 2000, Nature, 407:86-90.
Clark, A.J., 2000, Gene targeting in livestock: a preview, Transgenic Research, 9:263-275.
Capecchi, 1994, Targeted Gene Replacement, Scientific American, vol. 270, pp. 34-41.
http://en.wikipedia.org/wiki/Livestock.
Harrison, SJ et al., 2002, Efficient generation of 1(1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer, Transgenic Research, 11:143-150.
Poljaeva I.A.and Campbell, KHS, 2000, New advances in somatic cell nuclear transfer:application in transgenesis, Theriogenology, 53:117-126.
Thomson, AJ et al, 2003, Gene targeting in livestock, Reproduction Supplement, 61:495-508.
Akhmedov et al. “Characterization of two nuclear mammalian homologous DNA-pairing activities that do not require associated exonuclease activity”Proc. Natl. Acad Sci.USAvol. 92, pp. 1729-1733, Feb. 1995.
Liu et al., “Insulin-Like Growth Factor-I Affects Perinatal Lethality and Postnatal Development in a Gene Dosage-Dependent Manner: Manipulation Using the Cre/loxP System In Transgenic Mice”Molecular Endocrinology(1998), vol. 12, No. 9, 1452-1462.
Plug et al., “Presynaptic associate of Rad51 protein with selected sites in meiotic chromatin”Proc. Natl. Acad. Sci. USAvol. 93, pp. 5920-5924, Jun. 1996.
Utomo, A.R.H. “Temporal, Spatial, and Cell Type Specific Control of Cre-Mediated DNA Recombination in Transgenic mice”Nature Biotechnology, Nov. 1999, vol. 17, 1091-1098.

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