Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1998-12-01
2000-07-25
Fox, David T.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435447, 435440, C12N 1582, C12N 1501, C12N 1500
Patent
active
060935715
ABSTRACT:
A specific gene or DNA can be directly transferred into the pollen of a plant by selectively irradiating the shell of the pollen with ion beams without affecting the cell nucleus and thereafter immersing the irradiated pollen in a solution containing the specific gene or DNA of interest. The shell of the pollen of a plant may be irradiated with ions having a linear energy transfer (LET) of 5-10,000 keV/.mu.m to a controlled depth of ion injection. Hybridizing with pollen into which the specific gene or DNA has been transferred allows the specific gene to be transferred into a fertile embryo, thereby creating a transgenic plant.
REFERENCES:
patent: 5049500 (1991-09-01), Arnizen et al.
Tanaka et al., Beam Interactions with Materials & Atoms, Nuclear Instruments and Methods in Physics Research B 129, 42-48, 1997.
Potrykus, Gene Transfer to Cereals: An Assessment, Bio/Technology, pp. 535-542, 1990.
Tanaka et al., "Beam Interactions with Materials & Atoms," Nuclear Instruments and Methods in Physics Research B 129, 42-48 (1997).
Inoue Masayoshi
Tanaka Atsushi
Watanabe Hiroshi
Fox David T.
Grunberg Anne Marie
Japan Atomic Energy Research Institute
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