Refrigeration – Liquid contacting discrete commodity
Patent
1983-10-26
1984-12-11
Capossela, Ronald C.
Refrigeration
Liquid contacting discrete commodity
62430, 62514R, F25D 1702
Patent
active
044870337
ABSTRACT:
A method of freezing fertilized ova, spermatozoa or the like which has the steps of containing fertilized ova, spermatozoa or the like in a buffer solution at an irregular position in a tube, and cooling the same so that a buffer solution contained in the area containing no fertilized ova, spermatozoa or the like become lower in temperature than the buffer solution containing the fertilized ova, spermatozoa or the like with a desired refrigerant to freeze the second buffer solution to produce crystalline nuclei, and then cooling the nuclei so that the nuclei grow to the first buffer solution. And, an apparatus for freezing fertilized ova, spermatozoa or the like which can carry out the above method. Thus, the survival rate of the fertilized ova, spermatozoa or the like can be raised, and the fertilized ova, spermatozoa or the like can be readily frozen under an automatic control by simple cooling means.
REFERENCES:
patent: 3943993 (1976-03-01), Smith
patent: 4059967 (1977-11-01), Rowe et al.
patent: 4155331 (1979-05-01), Lawrence et al.
patent: 4199022 (1980-04-01), Senkan et al.
patent: 4232453 (1980-11-01), Edalmann
patent: 4377077 (1983-03-01), Granlund
Kuraoka Yasuo
Sakao Nobuo
Capossela Ronald C.
Hoxan Corporation
Oujevolk George B.
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