Refrigeration – Processes – Treating an article
Patent
1994-07-13
1995-09-26
Kilner, Christopher
Refrigeration
Processes
Treating an article
62 78, 62 511, F25D 1702, F24F 316
Patent
active
054525845
ABSTRACT:
Disclosed is an improved method for freezing biological specimens, such as human tissues, animal tissues, and plant tissues. The specimens are frozen so that they may be presently examined with light microscopy, or first stored frozen at ultra low temperatures and then later examined with light microscopy. The specimens are prepared with a matrix gel and then frozen with a fluorinated carbon fluid that is non-toxic to humans and thus the method does not present adverse health effects to humans. Preferably, the fluid also is non-flammable. More preferably, the fluid also has an ozone depletion potential of 0, whereby the preferred procedure is free of detrimental effects on the protective ozone layer of the earth. A suitable fluorinated carbon fluid is one that stays in the liquid state at low temperatures, for instance -60.degree. C. or lower. A suitable material is the perfluorinated carbon fluid, C.sub.6 F.sub.14.
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Duke University
Jenkins Richard E.
Kilner Christopher
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