Method of fracturing using mannanohydrolase enzyme breaker

Details Method of fracturing using mannanohydrolase enzyme breaker Method of fracturing using mannanohydrolase enzyme breaker

2009-10-15

2011-11-15

Nashed, Nashaat (Department: 1656)

Earth boring, well treating, and oil field chemistry

Well treating

Contains enzyme or living micro-organism

C435S267000

Reexamination Certificate

active

08058212

ABSTRACT:
A thermophilic mannanohydrolase enzyme may be used as an enzyme breaker for fracturing fluids containing hydratable polymers of guar and underivatized guar. The enzyme is effective in downhole temperatures exceeding 160° F.

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Daniel D. Morris et al., “Correction of the β-Mannanase Domain of thecelCPseudogene fromCaldocellulosiruptor saccharolyticusand Activity of the Gene Product on Kraft Pulp”, Applied and Environmental Microbiology, Jun. 1995, vol. 61, No. 6, pp. 2262-2269.
Database UniProt [Online] Mar. 24, 2009, “SubName: Full=Mannan endo-1,4-beta-mannosidase., Cellulase; EC=3.2.1.4; EC=3.2.1.78; Flags: Precursor;”, retrieved from EBI accession No. UNIPROT:B9MKU6.
Database UniProt [Online] May 1, 2000, “SubName: Full=Multidomain beta-1,4-beta-mannanase; Flags: Precursor;”, retrieved from EBI accession No. UNIPROT:Q9RFX5.
Lüthi, E., et. al.; Cloning, Sequence Analysis, and Expression inEscherichia coliof a Gene Coding for a β-Mannanase from the Extremely Thermophilic Bacterium “Caldocellum saccharolyticum;” Applied and Environmental Microbiology; Mar. 1991; pp. 694-700; American Society for Microbilogy.

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