Method of fractionating and separating proteins

Details Method of fractionating and separating proteins Method of fractionating and separating proteins

1977-05-11

1978-08-29

Danison, Walter C.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

A23J 100

Patent

active

041103203

ABSTRACT:
Improvement in a method for the fractionation and separation of proteins by dsorption on an organic gel and elution with a descending ammonium sulfate gradient, the improvement comprising treating a solution of the proteins with ammonium sulfate down to a concentration which is from 5 to 15% below the concentration normally required for protein precipitation, mixing the solution obtained with unsubstituted agarose gel, whereby the protein is precipitated onto the agarose gel, and then fractionally eluting the latter, preferably with a linearly descending gradient.

REFERENCES:
raibaud et al., FEBS Letters, 50, 130-134 (1974).
Shaltiel et al., Proc. Nat. Acad. Sci., U.S.A., 72, 3397-3401 (1975).

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