Method of forming signal probe-polymer

Details Method of forming signal probe-polymer Method of forming signal probe-polymer

2011-04-19

2011-04-19

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S007100, C435S007200, C536S024300, C536S024330, C536S025300, C536S026600

Reexamination Certificate

active

07927804

ABSTRACT:
Provided are a method of forming a signal probe-polymer which makes it possible to form a polymer efficiently and quantitatively, a signal probe-polymer formed by the method, oligonucleotide probes for use in the method, and a method of detecting target analyte having high sensitivity and excellent quantitative capability. The method of forming a signal probe-polymer comprises reacting a plurality of pairs of oligonucleotide probes with each other to form a polymer, a first probe of the pair of oligonucleotide probes comprising three nucleic acid regions of X, Y, and Z regions, located in the stated order from the 5′-terminal and a second probe comprising three nucleic acid regions of X′, Y′, and Z′ regions, located in the stated order from the 5′-terminal, wherein each region of the oligonucleotide probes has a length of from 13 to 15 bases.

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European Office Action issued Aug. 26, 2010 in corresponding European Patent Application No. 07 73 8507.

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