Method of forming planar membrane

Coating processes – Foraminous product produced – Filter – sponge – or foam

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427299, 427387, 424450, 436829, 530359, 435174, 435177, 264215, 264212, 264299, G01N 33545, C07K 1708, B05D 500

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052885178

ABSTRACT:
A method of forming a lipid planar membrane of a bilayer membrane composed of lipid and protein comprises the step of immersing a substrate, which has a long-chain hydrocarbon group at least on the surface of one side thereof, in a suspension of liposome or proteoliposome, respectively. The long-chain hydrocarbon group is introduced into the substrate by bonding a long-chain alkylsilane coupling agent or a long-chain alkyltitanate coupling agent.

REFERENCES:
Ishiguro, T. "Preparation of Supported Membranes Containing Transmembrane Proteins." J. Biochem., vol. 95 (1984) pp. 581-583.
Weis et al. "Stimulation of Fluorescence in a Small Contact Region Between Rat Basophil Leukemiacens and Planar LIPID Membrane Target by Coherent Evanscent Radiation." The Journal of Biological Chemistry, vol. 257 (1982) pp. 6440-6445.
Horio and Yamashita, "Tanpakushitsu Kosono Kisojikkenho (Basic Experimental Methods for Proteins and Enzymes)," 1981, pp. 147-149, Nankodo Co.
SPOP Heterobifunctional Reagent, Sweden, 1978, pp. 1-12 (Pharmacia Fine Chemicals).
Deamer, D. W. and Uster, P.S. "Liposome Preparation: Methods and Mechanisms." in: Osto, M. J., Liposomes (New York and Basel, Marcel Dekker, Inc. 1983), pp. 31-32.
Gruner, S. M. "Materials Properties of Liposomal Bilayers." in: Ostro, M. J., Liposomes from Biophysics to Therapeutics (New York and Basel, Marcel Dekker, Inc. 1987), pp. 4-5.
The Condensed Chemical Dictionary, 8th ed. New York, van Nostrand Reinhold Company, 1971 pp. 641 and 380.
Stryer, L. Biochemistry, USA., W. H. Freeman and Company/New York, 1988, pp. 289-290.
Chemical Abstract Data Sheet of CA;109(25) 225157a. Dec. 1988.

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