Method of forming a two-dimensional distribution image of ion co

Details Method of forming a two-dimensional distribution image of ion co Method of forming a two-dimensional distribution image of ion co

1990-08-06

1992-03-17

Hannaher, Constantine

Radiant energy

Luminophor irradiation

With ultraviolet source

2504591, 2504612, 436172, G01N 2164

Patent

active

050971352

ABSTRACT:
This invention relates to a method of forming a two-dimensional concentration distribution image of specific ions to be assayed in a living cell, on the basis of the variation in fluorescent spectrum or excitation spectrum of a fluorescent probe. This method comprises the steps of administering a fluorescent probe into a cell, obtaining a fluorescence intensity distribution image by determining fluorescence light from the fluorescent probe in a region including the cell, measuring the cell optically, thereby obtaining a cell image of the cell selected from the group consisting of a bright-field image, a phase contrast image, a Nomarski differential interference contrast image, and a polarization image, determining a background fluorescence value from a profile of the fluorescence intensity image and a profile of the cell image, forming a two-dimensional concentration distribution image of the ions to be assayed, by image processing including a subtraction of the background fluorescence value from the fluorescence intensity distribution image, and subjecting the cell image and the two-dimensional concentration distribution image of the ions to image processing, thereby displaying both images in an overlapping manner.

REFERENCES:
patent: 4006360 (1977-02-01), Mueller
patent: 4492752 (1985-01-01), Hoffman et al.
patent: 4495293 (1985-01-01), Shaffer
patent: 4604520 (1986-08-01), Pohl
patent: 4631750 (1986-12-01), Gabriel et al.
patent: 4668868 (1987-05-01), Noller
Nature, vol. 318, Dec. 12, 1985, pp. 558-561, Williams et al., "Calcium Gradients in Single Smooth Muscle Cells Revealed by Digital Imaging Microscope Using Fura-2".

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