Method of fluorometrically measuring the activity of fat-degradi

Details Method of fluorometrically measuring the activity of fat-degradi Method of fluorometrically measuring the activity of fat-degradi

1984-02-22

1987-05-26

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435 21, C12Q 144

Patent

active

046686231

ABSTRACT:
The object of the present invention is a new method for fluorometrically assaying the activity of fat-degrading enzymes, such as lipases and phospholipases in samples containing said enzyme, such as in serum. According to the method the enzyme containing sample is reacted with a substrate containing acyl- or alkylglycerols or -phosphoglycerols having at least one fluorescent group, such as a pyrene group. The compounds may in addition contain flurorescence quenching groups. The enzyme hydrolyzes the substrate thus giving rise to changes in the fluorescence intensity during the enzyme reaction and the changes in the intensity are measured at a specific emission wavelength of the fluorescent group employed. The rate of change of the invensity is proportional to the enzyme activity in the sample.
The invention concerns also the new compounds containing fluorescent groups to be used in the method.

REFERENCES:
patent: 4261968 (1981-04-01), Ullman et al.
patent: 4360694 (1982-11-01), Kinnunen et al.
Fleisher et al.--Chem. Abst., vol. 75 (1971), p. 105296c.
Taskinen et al.--Clinic Chem. Acta, vol. 104 (1980), pp. 107-117.

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