Radiant energy – Luminophor irradiation – Methods
Reexamination Certificate
2011-03-01
2011-03-01
Porta, David P (Department: 2884)
Radiant energy
Luminophor irradiation
Methods
Reexamination Certificate
active
07897937
ABSTRACT:
For imaging a structure in a sample with spatial resolution, the structure is labeled with a fluorophore which is transferable by an optical transfer signal out of a first into a second photochromic state. Via a common objective, the sample is subjected to both the focussed optical transfer signal and a focussed optical excitation signal only exciting a portion of the fluorophore being in its second photochromic state for fluorescence. The transfer and the excitation signal have a common centre of maximum intensity; and a decrease of intensity of the transfer signal with the distance to this common centre is substantially stronger than any decrease of the effective return rate of the fluorophore back into the first photochromic state. Fluorescence light emitted by the excited fluorophore is detected. Then, the common centre is shifted with regard to the sample; and the steps of subjecting and detecting are repeated.
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Engelhardt Johann
Hell Stefan
Lang Marion
Deutsches Krebsforschungszentrum
Kim Kiho
Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.
Porta David P
Thomas Kayden Horstemeyer & Risley LLP
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