Method of extracting virus from cell culture

Details Method of extracting virus from cell culture Method of extracting virus from cell culture

2007-03-06

2007-03-06

Chen, Stacy B. (Department: 1648)

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

C424S238100, C424S239100, C424S215100

Reexamination Certificate

active

10916378

ABSTRACT:
The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25° C. or 37° C. to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.

REFERENCES:
patent: 6146873 (2000-11-01), Kistner et al.
patent: 6194191 (2001-02-01), Cho et al.
patent: 6528305 (2003-03-01), Thompson et al.
patent: 63044532 (1988-02-01), None
patent: 99/08692 (1999-02-01), None
patent: 02/12435 (2002-02-01), None
Berry et al., “Production of Reovirus Type-1 and Type-3 from Vero Cells Grown on Solid and Macroporous Microcarriers”,Biotechnology and Bioengineering62: 12-19, 1999.
Bos, “Ras Oncogenes in Human Cancer: A Review”,Cancer Research49(17): 4682-4689, 1989.
Chandron & Nibert, “Protease cleavage of reovirus capsid protein μ1 and μ1C is blocked by alkyl sulfate detergents, yielding a new type of infectious subvirion particle”,J. of Virology72(1): 467-75, 1998.
Coffey et al., “Reovirus therapy of tumors with activated Ras pathway”,Science282: 1332-1334, 1998.
Drastini et al., “Comparison of eight different procedures for harvesting avian reoviruses grown in Vero cells”,J. Virological Methods39: 269-278, 1992.
Drayna et al., “Genetic Studies on the Mechanism of Chemical and Physical Inactivation of Reovirus”J General Virology63(1): 149-160, 1982.
Hitt et al., “Construction and propagation of human adenovirus vectors”,Cell Biology1(3): 500-512, 1998.
McCrae, “The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3”,Virology, 89: 578-593, 1979.
Mendez et al., “A comparative analysis of Freon substitutes in the purification of reovirus and calicivirus”,J. Virological Methods90(1): 59-67, 2000.
Mora et al., “Association of Reovirus Proteins with the Structural Matrix of Infected Cells”,Virology159(2): 265-277, 1987.
Nibert et al., “Reoviruses and their replication”,Fundamental VirologyThird Edition, 691-730, 1996.
Smith, R.E. et al., “Polypeptide components of virions, top component and cores of reovirus type 3”,Virology39: 791-810, 1969.
Strong et al., “The v-erb V oncogene confers enhanced cellular susceptibility to reovirus infection”,J. Virol.70: 612-161, 1996.
Strong et al., “Evidence that the Epidermal Growth Factor Receptor on Host Cells Confers Reovirus Infection Efficiency”,Virology197(1): 405-411, 1993.
Strong et al., “The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus”,EMBO J.17: 3351-3362, 1998.
Taber, “The selection of virus-resistant Chinese hamster ovary cells”,Cell8: 529-533, 1976.

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