Method of extending the linear range of an enzyme immunoassay by

Details Method of extending the linear range of an enzyme immunoassay by Method of extending the linear range of an enzyme immunoassay by

1987-06-05

1990-04-03

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 21, 436500, 436518, 436536, 436548, 436818, G01N 3353, G01N 33543, G01N 33536

Patent

active

049140230

ABSTRACT:
The present invention relates to enzyme immunoassays employing one or more enzyme label-substrate pairs which give rise to colored products and in which absorption by the product of substrate conversion by at least one enzyme label at a first wavelength, at or close to the optimum wavelength for absorbance, exceeds the linear range of the detector, which immunoassay includes the steps of measuring the said substrate conversion at a second wavelength at which absorbance by the relevant product is significantly lower than at the said first wavelength and calculating the true absorbance at the said first wavelength by utilizing the result of linear regression analysis of absorbance measurements obtained with product standards at the said first and second wavelengths within the linear range of the detector.

REFERENCES:
patent: 4496658 (1985-01-01), Kondo et al.
patent: 4672028 (1987-06-01), Olson
patent: 4690890 (1987-09-01), Loor et al.
"Methods of Enzymatic Analysis", 3rd Edition, vol. X, Antigens and Antibodies 1, VCH Publishers, Weinheim, FRG, May 1986, Bergmeyer, editor.
"Clinical Diagnosis and Management by Laboratory Methods" J. B. Henry, editor; Saunders, Philadelphia, 1979, p. 86.

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