Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1997-08-05
Zitomer, Stepanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 681, 935 19, 935 81, C12Q 168, C12P 2106
Patent
active
056541503
ABSTRACT:
The present invention provides improvements in the expression cloning method for isolating novel cDNA clones. In particular, in the expression cloning method comprising the steps of preparing a cDNA library from a cell that expresses a desired protein; inserting the cDNA library into an expression vector; inserting the cDNA library into bacterial cells and culturing the bacterial cells to produce individual bacterial colonies; collecting pools of a predetermined number of individual bacterial colonies; isolating the cDNAs contained in the pools; expressing proteins encoded by the cDNAs; and detecting the desired protein, positive clones may be efficiently and cost-effectively obtained by collecting pools of about 100 individual bacterial colonies and expressing proteins encoded by the cDNAs in the pools in vitro.
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King Randall W.
Kirschner Marc W.
Lustig Kevin D.
Stukenberg P. Todd
Atzel Amy
Harvard University, Office of Technology and Trademark Licensing
President and Fellows of Harvard College
Zitomer Stepanie W.
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