Method of expressing genes in mammalian cells

Details Method of expressing genes in mammalian cells Method of expressing genes in mammalian cells

1993-11-04

1998-09-08

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

935 34, C12P 2105

Patent

active

058044078

ABSTRACT:
The present invention relates to a method of expressing a heterologous gene in mammalian cells and a recombinant DNA construct for use in the method. The invention also relates to a method of specifically killing cells which constitutively express AFP.

REFERENCES:
patent: 5206153 (1993-04-01), Tamaoki et al.
Ledley, Human Gene Therapy 2: 77 (1991).
Friedmann, Cancer Supplement 70: 1810 (1992).
Wen et al, DNA Cell Biol. 10: 525 (1991).
Hodgson, Bio/Technology 13: 222 (1995).
Anderson, W.F. (1984) "Prospects for Human Gene Therapy," Science, 226:401-409.
Behringer et al. (1988) "Dwarf Mice Produced by Genetic Ablation of Growth Hormone-Expressing Cells," Genes. & Develop., 2:453-461.
Breitman et al. (1987) "Genetic Ablation: Targeted Expression of a Toxin Gene Causes Microphthalmia in Transgenic Mice," Science, 238:1563-1565.
Breitman et al. (1990) "Genetic Ablation in Transgenic Mice with an Attenuated Diphtheria Toxin A Gene," Molecular and Cellular Biology, 10:474-479.
Giannini et al. (1984) "The Amino-Acid Sequence of Two Non-Toxic Mutants of Diphtheria Toxin: CRM45 and CRM197," Nucl. Acids Res., 12:4063-4069.
Gibbs et al. (1987) "Structure, Polymorphism, and Novel Repeated DNA Elements Revealed by a Complete Sequence of the Human .alpha.-Fetoprotein Gene," Biochemistry, 26:1332-1343.
Goring et al. (1987) "In Situ Detection of .beta.-Galactosidase in Lenses of Transgenic Mice with a .gamma.-Crystallin/lacZ Gene," Science, 235:456-458.
Graham et al. (1973) "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA," Virology, 52:456-467.
Hamasaki et al. (1992) "Interaction of Interferon-.alpha. with Interleukin-1.beta. or Tumor Necrosis Factor-.alpha. on Hepatitis B Virus Enhancer Activity" Biochem. Biophys. Res. Comm., 183:904-909.
Hashimoto et al. (1992) "A New Family of Homeobox Genes Encoding Multiple Homeodomain and Zinc Finger Motifs," Mech. Develop., 39:125-126.
Hatano et al. (1992) "Hepatocyte Growth Factor Down-Regulates the .alpha.-Fetoprotein Gene Expression in PLC/PRF/5 Human Hepatoma Cells," Biochemical and Biophysical Research Communications, 189:385-391.
Hayashi et al. (1992) "Identification and Characterization of Two Enhancers of the Human Albumin Gene," The Journal of Biological Chemistry, 267:14580-14585.
Huber et al. (1991) "Retroviral-Mediated Gene Therapy for the Treatment of Hepatocellular Carcinoma: An Innovative Approach for Cancer Therapy," Proc. Natl. Acad. Sci. USA, 88:8039-8043.
Kew, Michael C. (ed.) (1984) "Toward an Understanding of the Etiology of Hepatic Tumors," Goldfarb, Stanley Biologic Mechanisms of Hepatocarcinogenesis, Sherman et al. Hepatitis B Virus and Hepatocellular Carcinoma: Molecular Biology and Mechanistic Considerations, Beasley et al. Hepatocellular Carcinoma and Hepatitis B Virus, Newberne, Paul M. Chemical Carcinogenesis: Mycotoxins and Other Chemicals to Which Humans Are Exposed, Kew et al. Relationship Between Hepatocellular Carcinoma and Cirrhosis, Mays and Christopherson Hepatic Tumors Induced by Sex Steroids, Tamburro, Carlo H. Relationship of Vinyl Monomers and Liver Cancers: Angiosarcoma and Hepatocellular Carcinoma, Foster, James H. Treatment of Metastatic Disease of the Liver: A Skeptic's View, Seminars in Liver Disease, 4:iii-v and 89-179.
Lopata et al. (1984) "High-level Transient Expression of a Chloramphenical Acetyl Transferase Gene by DEAE-Dextran-Mediated DNA Transfection Coupled With a Dimethyl Sulfoxide or Glycerol Shock Treatment," Nucl. Acids Res., 12:5707-5717.
McCormick, D. (1985) "Human Gene Therapy: The First Round," Bio/Technology, 3:689, 691-693.
Mitsuoka et al. (1992) "Inhibitory Effect of Prostaglandin .DELTA..sup.12 -PGJ2 on Cell Proliferation and .alpha.-Fetoprotein Expression in HuH-7 Human Hepatoma Cells," Prostaglandins, 43:189-197.
Morinaga et al. (1983) "Primary Structures of Human .alpha.-Fetoprotein and its mRNA," Proc. Natl. Acad. Sci. USA, 80:4604-4608.
Morinaga et al. (1991) "A Human .alpha.-Fetoprotein Enhancer-Binding Protein, ATBF1, Contains Four Homeodomains and Seventeen Zinc Fingers," Mol. Cell. Biol., 11:6041-6049.
Moro et al. (1993) "Monoclonal Antibodies Directed Against a Widespread Oncofetal Antigen: The Alpha-Fetoprotein Receptor," Tumor Biol., 14:116-130.
Nakabayashi et al. (1989) "Transcriptional Regulation of .alpha.-Fetoprotein Expression by Dexamethasone in Human Hepatoma Cells," The Journal of Biological Chemistry, 264:266-271.
Nakabayashi et al. (1991) "A Position-Dependent Silencer Plays a Major Role in Repressing .alpha.-Fetoprotein Expression in Human Hepatoma," Molecular and Cellular Biology, 11:5885-5893.
Nakabayashi et al. (1992) "Selective Expression of a Toxin Gene Under the Direction of Human .alpha.-Fetoprotein Gene Regulatory Sequences in the Human Hepatoma Cells--An Approach to Hepatocellular Carcinoma Gene
Nakao et al. (1989) "Involvement of an AFP1-Binding Site in Cell-Specific Transcription of the Pre-S1 Region of the Human Hepatitis B Virus Surface Antigen Gene," Nucleic Acids Research, 17:9833-9842.
Nakao et al. (1990) "c-Ha-ras Down Regulates the .alpha.-Fetoprotein Gene but not the Albumin Gene in Human Hepatoma Cells," Mol. Cell. Biol., 10:1461-1469.
Nakao et al. (1991) "Transforming Growth Factor .beta.1 Differentially Regulates .alpha.-Fetoprotein and Albumin in HuH-7 Human Hepatoma Cells," Biochemical and Biophysical Research Communiccations, 174:1294-1299.
Nakata et al. (1992) "A Possible Mechanism of Inverse Developmental Regulation of .alpha.-Fetoprotein and Albumin Genes," The Journal of Biological Chemistry, 267:1331-1334.
Nerenstone et al. (1988), "Clinical Trials in Primary Hepatocellular Carcinoma: Current Status and Future Directions," Cancer Treatment Reviews, 15:1-31.
Neumann et al. (1982) "Gene Transfer into Mouse Lyoma Cells by Electroporation in High Electric Fields," EMBO J. 1:841-845.
Otsuru et al. (1988) "Analysis of Alpha-Fetoprotein Gene Expression in Hepatocellular Carcinoma and Liver Cirrhosis by in Situ Hybridization," Cancer, 62:1105-1112.
Palmiter et al. (1987) "Cell Lineage Ablation in Transgenic Mice by Cell-Specific Expression of a Toxin Gene, Cell", 50:435-443.
Pappenheimer, A.M. Jr. (1977) "Diphtheria Toxin," Ann. Rev. Biochem., 46:69-94.
Potter et al. (1984) "Enhancer-Dependent Expression of Human K Immunoglobubin Genes Introduced into Mouse Pre-B Lymphocytes by Electroporation," Proc. Natl. Acad. Sci. U.S.A., 81:7161-7165.
Sakai et al. (1985) "The Human .alpha.-Fetoprotein Gene: Sequence Organization and the 5' Flanking Region," The Journal of Biological Chemistry, 260:5055-5060.
Sawadaishi et al. (1988) "Interaction of a Hepatome-Specific Nuclear Factor with Transcription-Regulatory Sequences of the Human .alpha.-Fetoprotein and Albumin Genes," Molecular and Cellular Biology, 8:5179-5187.
Tamoki, T. (1991) "Alpha-Fetoprotein as a Tumor Marker: Thoughts on the Molecular Mechanism for Controlling the Gene Expression." Kagaku To
Tsutsumi et al. (1993) "Regulation of Albumin and .alpha.-Fetoprotein Gene Expression by Colloid Osmotic Pressure in Human Hepatoma Cells," Gastroenterology, 104:256-262.
Uchida et al. (1973) "Diphtheria Toxin and Related Proteins I. Isolation and Properties of Mutant Proteins Serologically Related to Diphtheria Toxin," J. Biol. Chem., 248:3838-3844.
Urano et al. (1984) "Tandem Arrangement of the Albumin and .alpha.-Fetoprotein Genes in the Human Genome," Gene, 32:255-261.
Urano et al. (1986) "The Human Albumin Gene: Characterization of the 5' and 3' Flanking Regions and the Polymorphic Gene Transcripts," The Journal of Biological Chemistry, 261:3244-3251.
Urano et al. (1991) "Interstitial Chromosomal Deletion within 4q11-q13 in a Human Hepatoma Cell Line," Cancer Res., 51:1460-1464.
Watanabe et al. (1987) "Cell-Specific Enhancer Activity in a Far Upstream Region of the Human .alpha.-Fetoprotein Gene," The Journal of Biological Chemistry, 262:4812-4818.
Wong and Neumann (1982) "Electric Field Mediated Gene Transfer," Biochem. Biophys. Res. Comm., 107:584-587.
Wu et al. (1985) "Acetaminophen Hepatotoxicity and Targeted Rescue: A Model for Specific Chemotherapy of Hepatocellular C

Affiliated with

Also associated with

Rating

Method of expressing genes in mammalian cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of expressing genes in mammalian cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of expressing genes in mammalian cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1281142