Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-06-02
2000-03-07
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Vector, per se
435 694, 435 691, 435 697, 435 698, 435325, 435348, 435455, 435456, 530398, 530397, 536 2351, C12N 1500
Patent
active
060339039
ABSTRACT:
The extracellular domain, or fragment thereof, of a gonadotropin glycoprotein hormone receptor is expressed and secreted in a soluble and functionally hormone-binding form. A recombinant baculovirus transfer vector is constructed to include a gene segment encoding the extracellular domain, or fragment thereof, of the glycoprotein hormone receptor joined in frame with a gene segment encoding a baculovirus signal peptide and operably linked to a baculovirus promoter. Recombinant baculovirus generated by transfection or co-transfection of insect cells are then used to infect insect host cells for the expression and secretion of soluble receptor.
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Buckler David Rogers
Cheng Shirley Vui Yen
Prentice Holly Lynn
Sisk William P.
Applied Research Systems Ars Holding N.V.
Hutzell Paula K.
Lazar-Wesley Eliane
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