Method of expanding hepatic precursor cells

Details Method of expanding hepatic precursor cells Method of expanding hepatic precursor cells

1994-06-24

1996-11-19

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524021, 43524023, C12N 500, C12N 506, C12N 508

Patent

active

055762077

ABSTRACT:
A composition which comprises an animal cell population, and which contains immature animal cells. The immature animal cells are characterized by expression of alpha-fetoprotein or lack of essential expression of alpha-fetoprotein and albumin, and at least a portion of said immature animal cells or at least a portion of the progeny of said immature cells is capable of differentiating into cells which express albumin. The cell population is cultured under conditions which result in expansion of the cells. Expansion of the cells may be achieved by culturing the cells in the presence of an extracellular matrix and liver stromal cells; and preferably in the presence of growth factors. Such cells may be used for liver transplantation, artificial livers, and for toxicology and pharmacology studies. Such cells may also be genetically engineered to express proteins or polypeptides of interest.

REFERENCES:
patent: 5030105 (1991-07-01), Kuri-Harcuch et al.
Tsai, et al "Isolation of a human stromal cell strain secreting hemopoietic growth factors" Biol. Abstracts #22,434 83(2) 1986.
Hirata et al, "Effects of basement membrane matrix on the culture of fetal mouse hepatocytes" Biological Abstracts 57,933 77(8) 1983.
Maurer in Animal Cell Culture, Ed. by R. I. Freshney p. 23, 1986.
Enat et al. 1984. PNAS USA 81:1411-5.

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