Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing cells in suspension
Patent
1995-06-07
1998-03-17
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing cells in suspension
4352971, 435325, 435372, 435383, 435384, 435385, C12N 500, C12N 502, C12M 122
Patent
active
057285810
ABSTRACT:
The present invention provides methods and bioreactors for expanding stem cells in a population of cells substantially enriched in hematopoietic stem cells and substantially free of stromal cells. The method comprising the steps of inoculating the population of cells in an expansion container in a volume of suitable medium such that the cell density is at least about 5,000 cells/1 mL and at an initial oxygen concentration of less than 8%; adding an effective amount of at least one cytokine to cause stem cell expansion; culturing the cells under suitable conditions such that the cells condition the medium; increasing the oxygen concentration to about 20%; exchanging the medium at a rate which allows expansion of the stem cells; and culturing the cells under conditions such that the stem cells are expanded. The present invention also provides a bioreactor constructed to accomodate the operational requirements for stroma-free stem cell expansion.
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Chary Srikanth Ranga
Kuo Suzanne Chang
Schwartz Richard Merrill
Tucker Sean Newton
Lankford , Jr. Leon B.
Systemix, Inc.
Tate Christopher R.
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