Method of expanding and differentiating cord blood cells by...

Details Method of expanding and differentiating cord blood cells by... Method of expanding and differentiating cord blood cells by...

2005-04-25

2008-11-18

Saucier, Sandra E. (Department: 1651)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

Reexamination Certificate

active

07452662

ABSTRACT:
Based on previous evidence suggesting positive effects of fever on in vivo hematopoiesis, the effect of hyperthermia on the expansion and differentiation of megakaryocytes (MKs) in ex vivo cultures of CB CD34-enriched cells has now been tested. Cells were cultured at 37° C. or 39° C. for 14 days in cytokine conditions optimized for MK development, and analyzed periodically by microscopy, flow cytometry and colony assays. Compared to 37° C., cultures maintained at 39° C. produced much more total cells (5×), MK progenitors (9×) and total MKs (7×), and showed accelerated (3-4 days) and enhanced MK maturation with increased yields of proplatelets and platelets (11.7×). The increased number of CD34+ cells and myeloid progenitors in the 39° C. cultures also suggested a general stimulatory effect of hyperthermia on the expansion of more primitive stem/progenitor cells and of cells of other lineages.

REFERENCES:
patent: WO-00/28813 (2000-05-01), None
patent: WO-02/100898 (2002-12-01), None
Ashman et al., “Effect of incubation temperature on mitogen responses of lymphocytes from adult peripheral blood and from cord blood”, Clin. Exp. Immunol. 33 : 319-326 (1978).
Barnes et al., Cell Stress & Chaperones, No. 6, vol. 4, pp. 316-325, 2001.
Bruno et al., Haematologica, vol. 88, No. 4, pp. 379-387, 2003.
de Benedetti et al., The Journal of Biological Chemistry, vol. 261, No. 33, pp. 15800-15804, 1986.
Ferris et al., Natl. Acad. Sci. USA, vol. 85, pp. 3850-3854, 1988.
Gidáli et al., Stem Cells, vol. 18, No. 3, pp. 533-538, 1994.
Kanamaru et al., Stem Cells, vol. 18, No. 3, pp. 190-195, 2000.
Katschinski et al., Cancer Letters, No. 115, pp. 195-199, 1997.
Katschinski et al., Cytokine and Growth Factor Reviews, No. 10, pp. 93-97, 1999.
Kie et al., Stem Cells, No. 20, pp. 73-79, 2002.
Kim et al., Mol. Cells, vol. 14, No. 3, pp. 367-373, 2002.
Larocca et al., Int. J. Cancer, No. 73, pp. 75-83, 1997.
Milarski et al., Proc. Natl. Acad. Sci. USA, vol. 83, pp. 9517-9521, 1986.
Ménoret et al., International Journal of Hyperthermia, vol. 18, No. 6, pp. 490-505, 2002.
Pick et al., Exp. Hematol., vol. 30, No. 9, pp. 1079-1087, 2000.
Proulx et al., Journal of Hematotherapy & Stem Cell Research, No. 12, pp. 179-188, 2003.
Proulx et al., Biotechnol Bioeng., vol. 88, No. 6, p. 675-680, 2004.
Robins et al., Cancer Research, No. 48, pp. 6587-6592, 1988.
Robins et al., Journal of Clinical Oncology, vol. 11, No. 9, pp. 1787-1794, 1993.
Robins et al., Journal of Clinical Oncology, vol. 15, No. 1, pp. 158-164, 1997.
Robins et al., Cancer Letters, No. 97, pp. 195-201, 1995.
Sigurjónsson et al., Journal of Hematotherapy & Stem Cell Research, No. 11, pp. 389-400, 2002.
Su et al., Marrow Transplantation, No. 27, pp. 1075-1080, 2001.
Wierenga et al., Experimental Hematology, No. 31, pp. 421-427, 2003.
Kluger, M.J., et al., Annals New York Academy of Sciences, vol. 856, pp. 224-233, 1998.
Ashman, R.B., et al., Effect of incubation temperature on mitogen responses of lymphocytes from adult peripheral blood and from cord blood, Clin. exp. Immunol., vol. 33, pp. 319-326, (1978).
Shen, Rong-Nian et al., Influence of elevated temperature on natural killer cell activity, lymphokine-activated killer cell activity and lectin-dependent cytotoxicity of human umbilical cord blood and adult blood cells, International Journal of Radiation Oncology Biology Physics, vol. 29, No. 4, pp. 821-826, Database Biosis [Online], Biosciences Information Service, Philadelphia, PA, US; 1994, XP00246605.
Boucher, Jean-Francois et al., Identification of the mechanisms responsible for the increased megakaryopoiesis at 39 degrees C., vol. 108, No. 11, Part 1, Database Biosis [Online] , Biosciences Information Services, Nov. 2006, XP002466506.
Podesta, Marina et al., Modified in vitro conditions for cord blood-derived long-term culture-initiating cells, Experimental Hematology, vol. 29, pp. 309-314, 2001.

Affiliated with

Also associated with

Rating

Method of expanding and differentiating cord blood cells by... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of expanding and differentiating cord blood cells by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of expanding and differentiating cord blood cells by... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4022168