Method of etching islands of cells in a grid pattern

Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus

Reexamination Certificate

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C435S004000, C436S807000, C216S096000, C216S097000

Reexamination Certificate

active

10967703

ABSTRACT:
An apparatus and process for monitoring migratory cell proliferation with restricted migration on a substrate includes providing a substrate, coating the substrate with extracellular matrix, plating cells suspended in cell culture media on extracellular matrix, and placing intersecting channels across the extracellular matrix components by removing the extracellular matrix components from the channels to isolate islands of the extracellular matrix components on the substrate. When the cells are immersed with a fluid, migration of the cells is confined to the isolated islands of the extracellular matrix components, permitting long-term observation of a migratory population.

REFERENCES:
patent: 5976826 (1999-11-01), Singhvi et al.
patent: 6133030 (2000-10-01), Bhatia et al.
patent: 6368838 (2002-04-01), Singhvi et al.
patent: 7033821 (2006-04-01), Kim et al.
patent: 2003/0003571 (2003-01-01), Kanegasaki et al.
patent: WO 01/70389 (2001-09-01), None
Xia, Younan et al.; “Unconventional Methods for Fabricating and Patterning Nanostructures”; 1999,Chem. Rev., vol. 99, 1823-1848.

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