Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1991-11-25
1995-12-12
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 4, 435 27, C12Q 128, C12Q 126, C12Q 130, C12Q 100
Patent
active
054749085
ABSTRACT:
A method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.
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patent: 4587100 (1986-05-01), Amano et al.
patent: 4587245 (1986-03-01), Arai et al.
Ngo et al; "A Sensitive & Versatile Chromogenic Assay for Peroxidase & Peroxidase-Coupled Reactions", Jour. Analytical Biochem; vol. (105) No. 2, pp. 389-397, 1980.
White et al, Prin of Biochem; 6th Edition, Pub. 1978, pp. 419-421.
Saunders et al, "Peroxidase", Pub. 1964, p. 3.
Kurono Masayasu
Sawai Kiichi
Takehiro Osamu
Uno Shizuo
Leary Louise N.
Sanwa Kagaku Kenkyusho Co. Ltd.
Wityshyn Michael G.
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