Method of enhancing direct immunofluorescence staining of cells

Details Method of enhancing direct immunofluorescence staining of cells Method of enhancing direct immunofluorescence staining of cells

1990-04-27

1991-11-26

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 724, 435 77, 435 771, 435 78, 435 79, 435 34, 436519, 436548, 436800, 436801, 436824, 436828, G01N 33577

Patent

active

050681788

ABSTRACT:
A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired cells, comprising contacting a sample from the heterogeneous population of cells with a labeled primary antibody which recognizes and binds to a desired cell surface antigen and an unlabeled cross-linking agent which recognizes and binds to the primary antibody is disclosed.

REFERENCES:
patent: 4361550 (1982-11-01), Kung et al.
patent: 4381295 (1983-04-01), Kung et al.
F. M. Kourilsky et al., Europ. Jour. Immunol, 2, 249, 257, 1972.
J. J. Langone, Meth Enzymol, 70, 372, 1980.
R. L. Matthews et al., Jour. Immunol. Meth., 28, 219-232, 1979.
C. Neauport-Sautes et al., Jour. Exper. Med., 137, 511-526, 1973.
E. Soini, Clin. Chem., 25, 353-361, 1979.
Taylor et al., Nature New Biology, 233, 225-229, 1971.
Reinharz, E. L. & S. F. Schlossman, Cell 19: 821, 1980.
Cosimi et al., New England J. Medicine 306: 308, 1981.
Bach et al., Ann Immunol. 133: 131, 1982.
Johnson et al., Handbook of Experimental Immunology, D. M. Weir, Ed., Oxford: Blackwell Publications, 1979 (pp. 15.1-15.18).

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