Method of encapsulating cells in a tubular extrudate

Details Method of encapsulating cells in a tubular extrudate Method of encapsulating cells in a tubular extrudate

1993-12-17

1995-02-14

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

264 4, 264 47, 425382R, 435179, 435180, 43524022, 43524023, 435288, C12N 1104, C12N 500, C12M 140, B01J 1302

Patent

active

053895355

ABSTRACT:
Methods and systems are disclosed for encapsulating viable cells which produce biologically-active factors. The cells are encapsulated within a semipermeable, polymeric membrane by co-extruding an aqueous cell suspension and a polymeric solution through a common port to form a tubular extrudate having a polymeric outer coating which encapsulates the cell suspension. For example, the cell suspension and the polymeric solution can be extruded through a common extrusion port having at least two concentric bores, such that the cell suspension is extruded through the inner bore and the polymeric solution is extruded through the outer bore. The polymeric solution coagulates to form an outer coating. As the outer coating is formed, the ends of the tubular extrudate can be sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links.

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