Method of eliminating electrophoretic rate differentials

Details Method of eliminating electrophoretic rate differentials Method of eliminating electrophoretic rate differentials

1985-07-22

1987-05-26

Williams, Howard S.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

B01D 5702

Patent

active

046683623

ABSTRACT:
The disclosure relates to a method for substantially eliminating electrophoretic rate differentials between simultaneously disposed side-by-side lanes of test samples. The method includes the steps of defining and vertically orienting an elongate, solid rectangular volume of a host material having substantially uniform pore sizes, the material having a thin cross-section, the volume having a vertical axis aligned with the gravity vector, the rectangular volume having first and second vertical bases; embedding several test samples within a corresponding number of wells formed within an upper edge of the rectangular volume of host material; forming an electro-conductive fluid circuit across the vertical length of the volume of host material; applying an electrical potential in the range of 3,000 to 5,000 volts across the fluid circuit, the direction of the electrical potential being substantially in alignment with the gravity vector; providing the volume of host material with a vertical length in the range of 40 to 100 centimeters; and uniformly and rapidly controlling thermal values within the host material as the electrical potential is applied, to maintain the temperature of the host material in the range of 50 to 60 degrees Centigrade.

REFERENCES:
patent: 3677930 (1972-07-01), Meshbane et al.
patent: 3856655 (1974-12-01), Roberts
patent: 3980540 (1976-09-01), Hoefer
patent: 4142960 (1979-03-01), Hahn et al.
patent: 4224134 (1980-09-01), Hoefer et al.
patent: 4305799 (1981-12-01), Shwarz et al.
patent: 4374723 (1983-02-01), Vesterberg
Heftmann, E., Chromatography 2d Ed., Reinhold Publishing Co., New York, pp. 235-237 (1967).

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