Method of electrophoresis

Details Method of electrophoresis Method of electrophoresis

2006-12-12

2006-12-12

Nguyen, Nam (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S462000, C204S468000, C204S469000, C204S612000, C204S613000

Reexamination Certificate

active

07147762

ABSTRACT:
The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilized medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide-comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises reactive disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium.

REFERENCES:
patent: 4124638 (1978-11-01), Hansen
patent: 4698420 (1987-10-01), Urnovitz
patent: 4985128 (1991-01-01), Ebersole et al.
patent: 5159049 (1992-10-01), Allen
patent: 5238545 (1993-08-01), Yoshioka et al.
patent: 6638408 (2003-10-01), Speicher et al.

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