Method of effecting a change in a cell, and a vector

Details Method of effecting a change in a cell, and a vector Method of effecting a change in a cell, and a vector

2008-01-01

2008-01-01

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

C435S320100

Reexamination Certificate

active

07314737

ABSTRACT:
A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.

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