Method of distinguishing invasive E. coli that cause septicemia

Details Method of distinguishing invasive E. coli that cause septicemia Method of distinguishing invasive E. coli that cause septicemia

1986-02-25

1988-10-04

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

432 29, 432 34, C12Q 110

Patent

active

047756217

ABSTRACT:
A method for distinguishing invasive from noninvasive E. coli comprises collecting an undifferentiated bacterial sample from a host, contacting bacteria contained in the sample to a growth medium which contains a dye selectively taken up by invasive E. coli, incubating the growth medium to grow colonies of E. coli, and examining the growth medium to detect E. coli colonies which have taken up the dye. A two-step incubation procedure provides superior results. While the invention can be practiced with E. coli obtained from any host, specific procedures for practicing the invention in poultry are provided.

REFERENCES:
Shelley M. Payne and Richard A. Finkelstein, Infection and Immunity 18, 94 (1977).
E. K. Barbour, N. H. Nabbut and H. M. Al-Nakhli, Am. J. Vet. Res. 46, 989 (1985).
Panagiotis A. Daskaleros and Shelley M. Payne, Infection and Immunity 48, 165 (1985).
Anthony T. Maurelli, Barbara Blackmon and Roy Curtiss, III, Infection and Immunity 43, 397 (1984).
Edward E. Ishiguro, Teresa Ainsworth, Trevor J. Trust and William W. Kay, J. Bacteriol. 164, 1233 (1985).
Michael J. Surgalla and Earl D. Beesley, Applied Microbiology 18, 834 (1969).
J. Kaya Prpic, Roy M. Robins-Browne and R. Brent Davey, Journal of Clinical Microbiology 18, 486 (1983).
John J. Johnson and W. S. Chilton, Science 152, 1247 (1966).

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