Method of disinsertion of vitreous body from neural retina of th

Details Method of disinsertion of vitreous body from neural retina of th Method of disinsertion of vitreous body from neural retina of th

1995-04-24

1999-01-05

Weber, Jon P.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424 9461, 435200, 435201, 435202, A61K 3846, A61K 3847, C12N 924, C12N 926, C12N 928

Patent

active

058558837

ABSTRACT:
Chondroitinase II, a protein having an isoelectric point of approximately 8.4-8.45 and an apparent molecular mass of 112 kDa when electrophoresed in a 4 to 20% gradient acrylamide gel in 25 mM Tris/192 mM glycine buffer at pH 8.5 in the presence of about 0.1% (w/v) SDS, has been isolated and purified. A process for the copurification by affinity chromatography of the chondroitinase I and chondroitinase II proteins produced by Proteus vulgaris is also provided. The proteins can be further purified by metal chelating chromatography. Therapeutic or surgical compositions of isolated chondroitinases I and II or the copurified mixture of chondroitinase I and II are also disclosed. These compositions are used in a method for selectively and completely disinserting the vitreous body from the neural retina of an eye.

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