Method of discriminating between dyed particles and background f

Details Method of discriminating between dyed particles and background f Method of discriminating between dyed particles and background f

1976-01-02

1977-02-01

Dixon, Harold A.

Radiant energy

Luminophor irradiation

With ultraviolet source

250373, G01M 2138

Patent

active

040063607

ABSTRACT:
A system for discriminating between the fluorescent emission from dye molecules bound to biological particles and the background emission from free molecules of the dye in the solution suspending the particles, where the quantum efficiency of the bound dye molecules because of their bound state, differs from the quantum efficiency of the unbound dye so that the emission from the bound dye exhibits a longer statistical decay lifetime. The system involves irradiating the particles in the solution with a mode locked laser, and measuring fluorescent intensity in delayed synchronism with the mode locking frequency.

REFERENCES:
patent: 3886363 (1975-05-01), Ohnishi et al.
patent: 3918812 (1975-11-01), Holm
"Instrument To Measure Fluorescent Lifetime --" by Bennett, Review of Scientific Instruments, vol. 31, No. 12, Dec. 1960, pp. 1275-1279.
"Instrument To Measure Fluorescent Lifetimes --" by Brody, Review of Scientific Inst., vol. 28, No. 12, Dec. 1957, pp. 1021-1026.
"N(3-Pyrene) maleimide: A Long Lifetime Fluro. Sulfhydryl Reagent" by Weltman et al., Journal of Biological Chemistry, May 1973, pp. 3173, 3176.
"Lifetime-Separated Spectroscopy: --" by Pruett et al., Journal of Chemistry & Physics, vol. 62, No. 6, Mar. 15, 1975, pp. 2050-2056.
"Sensitized Fluorescence Measurements --" by Pace et al., Journal of Physics E: Scientific Instrument, 1974, vol. 7.
Fluoresence Assay in Biology & Medicine, vol. II, by Sidney Udenfriend, Academic Press, 1969, pp. 82, 83, 162, 163.

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