Method of directionally orienting plant embryos

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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Details

43524046, 43524054, 47 58, 47 576, C12N 500, C12N 502, A01C 100, A01C 106

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active

052847659

ABSTRACT:
The invention is a method of ordering plant embryos so that a specified end of the embryo is always oriented in a predetermined direction. The embryos are preferably first fairly rapidly desiccated to about 15% moisture content. They are then suspended in a benign liquid flotation medium having a density in the range of about 1.059-1.104 g/cm.sup.3. The density must be adjusted empirically so that a predominant number of viable embryos will float and nonviable embryos will sink. In at least the case of conifer somatic embryos, they will float with the end bearing the latent cotyledons upward. After sufficient separation time in the flotation medium the oriented embryos are swept by a flowing liquid stream into a conduit. They enter cotyledon end first and are then carried to a delivery point without losing that orientation. Here they are separated from the transporting medium. The embryos, still positioned cotyledon end first, may then be picked up by robotic or other means for further processing, such as insertion into an artificial seed.

REFERENCES:
patent: 4467560 (1984-08-01), Simak
Levin et al., Bio/Technology, vol. 6, pp. 1035-1040 (Sep. 1988).
Gray et al., Critical Reviews in Plant Science, 10(1) pp. 33-61 (1991).
Gray et al., In Vitro Cell & Dev. Biology, 23(1), pp. 29-33 (Jan. 1987).
Kim, Yong-Hwan, C. C. Velho, and J. Janick. Separation of somatic embryo stages by layered sucrose density gradient. HortScience, Programs and Abstracts (suppl.), p. 137 (1989).
Velho, C. C., Y. Saranga, and J. Janick. Density separation of zygotic and somatic embryos. HortScience, 25 (9): 1120 (1990). (Paper delivered Am. Soc. of Hort. Sci. Nov. 4-8, 1990, Tucson, Ariz.

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